Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Lipoxin Signaling in Murine Lung Host Responses to Cryptococcus neoformans Infection

doi: 10.1165/rcmb.2014-0102OC

Figure Lengend Snippet: Strain-specific cytokine production in response to lung Cne infection. Cytokine profiles of naive and Cne-infected (Day 14 after infection) C.B-17 (open bars) and C57BL/6 (solid bars) mice were measured in BALF by cytokine bead array (see Materials and Methods). Cytokines analyzed included IL-17 (A), IFN-γ (B), IL-4 (C), IL-13 (D), TNF-α (E), and IL-1β (F). Data are presented as mean ± SEM (n = 5 per group). *P ≤ 0.01 for comparisons between Day 0 versus Day 14 within a strain. #P ≤ 0.01 for comparisons between strains at a given time point. Data were analyzed by Student’s t tests for differences within and between strains at specific time points.

Article Snippet: Aliquots of bronchoalveolar lavage fluid (BALF) were submitted to Aushon for multiplex cytokine immunoassay (SearchLight Chemiluminescent Protein Array; Aushon, Billerica, MA).

Techniques: Infection

Journal: Science translational medicine

Article Title: Prevention of Alveolar Destruction and Airspace Enlargement in a Mouse Model of Pulmonary Lymphangioleiomyomatosis (LAM)

doi: 10.1126/scitranslmed.3003840

Figure Lengend Snippet: Increases in MMPs, inflammatory cells, and cytokines and decrease in alveolar elastin are associated with TSC2-null lung lesion growth. (A to C) MMP expression assessed in the cell-free supernatant of the BAL fluid at the indicated time points. A multiplex assay was performed by Searchlight technology (Aushon Biosystems). Data are means ± SE of n = 11 in each group. ***P < 0.001 for days 15 and 20 versus day 0 by ANOVA (Bonferroni-Dunn). (D) Schematic representation of elastin and collagen disposition in the alveolar neck. (E) Volumes of elastin (Eln) and collagen (Col) in alveoli of Control and TSC2-null lesion–carrying mice. Elastin and collagen were analyzed in alveoli necks of vehicle-injected (Contr) and TSC2-null cell–injected (TSC2-null) mice and expressed as percentage of the total volume of the alveolar neck. Data are means ± SE. **P < 0.01 for Control versus TSC2-null lesion–carrying mice by ANOVA (Bonferroni-Dunn). (F and G) Representative images of alveoli necks of vehicle-injected (Control) (F) and TSC2-null cell–injected (TSC2-null) (G) mice. Verhoeff's elastin stain and Picro-Ponceau counterstain were used to detect elastin (black) and collagen (red), respectively. Arrowheads, elastin-enriched areas. Scale bar, 10 μm. (H to L) Proinflammatory cytokine and chemokine expression assessed with a multiplex assay in the cell-free supernatant of BAL fluid from mice at the indicated time points after tumor inoculation. (M) Inflammatory cell counts assessed from the BAL collected at times as indicated after tumor cell inoculation. Data are means ± SE of n = 11 in each group. *P < 0.05; **P < 0.01; ***P < 0.001 versus day 0 by ANOVA (Bonferroni-Dunn).

Article Snippet: ELISA was performed with a Searchlight Protein Array multiplex of cell-free supernatant of the BAL fluids at Aushon Biosystems.

Techniques: Expressing, Multiplex Assay, Injection, Staining

Journal: Science translational medicine

Article Title: Prevention of Alveolar Destruction and Airspace Enlargement in a Mouse Model of Pulmonary Lymphangioleiomyomatosis (LAM)

doi: 10.1126/scitranslmed.3003840

Figure Lengend Snippet: Rapamycin plus simvastatin rescues animal survival, prevents lesion growth and lung destruction, and abrogates MMP induction. Mice injected with diluent (Control) or TSC2-null cells were treated with vehicle, rapamycin (Rapa), simvastatin (Simva), and rapamycin + simvastatin (Rapa + Simva) from day 3 after injection. (A) Weight of control (black) and TSC2-null cell–injected (red) mice were examined from day 3 (arrows) to day 20 of experiment. Data are means ± SE of n > 5 in each group. *P < 0.01 for Control versus TSC2-null cell–injected mice by ANOVA (Bonferroni-Dunn). Arrows, beginning of treatment. (B to D) H&E staining of murine lungs. Scale bar, 500 μm. (B) Lesion/lung ratio (C) and MAAA analysis (D) were performed at day 20 after injection. Data are means ± SE of n > 8 in each group. **P < 0.001 for Control versus TSC2-null cell–injected vehicle-treated mice and for Rapa, Simva, and Rapa + Simva versus vehicle for TSC2-null cell–injected mice by ANOVA (Bonferroni-Dunn). (E to G) Expression of MMP-2 (E), MMP-3 (F), and MMP-9 (G), assessed in the cell-free supernatant of BAL at day 20 by multiplex assay. Data are means ± SE of n > 6 in each group. **P < 0.001 for compound- versus vehicle-treated mice by ANOVA (Bonferroni-Dunn).

Article Snippet: ELISA was performed with a Searchlight Protein Array multiplex of cell-free supernatant of the BAL fluids at Aushon Biosystems.

Techniques: Injection, Staining, Expressing, Multiplex Assay